This paper investigates the potential for the non-visual effects of daylight in buildings. It is the second part of a study, where the first paper focused on the formulation of the photobiological underpinnings of a threshold-based model configured for lighting simulation from the perspective of the human non-visual system (e.g. circadian response). This model aims to establish a link between light exposure at the eye in an architectural context and expected effects on the non-visual system. The spectral character of daylight also becomes a sensitive factor in the magnitude of the predicted non-visual effect. A means to ‘condense’ non-visual effects into a synthesised graphical format for the year, split by periods of the day, is described in terms of how such a format could inform design decisions. The sensitivity of the simulation model’s predictions to prevailing climate and building orientation is demonstrated by comparing results from eight European locations.